Table 3. Transcytosis of Lucifer yellow (LY) in the presence of compounds affecting transcytosis of DNA-fragment.

Compound	Factor	Treated, %	Control, %	n	m	p
pUC19 (lin) (242 nM)	0.88	1.1	1.2	2	12	0.5473
CpG ODN (10 µM)	3.1	3.7	1.2	3	17	0.0260
Fucoidan (100 µg/ml)	0.88	1.1	1.2	2	12	0.4730
Heparin sulphate (200 µg/ml)	0.84	1.0	1.2	2	12	0.4691
Dextran sulphate (250 µg/ml)	0.66	0.27	0.41	3	17	0.4317
CytD (2.5 µg/ml)	5.7	5.5	0.97	7	40	<0.001

Transcytosis of LY in the apical to basolateral direction with (treated) and without (control) competitor was quantified after 90 minutes of incubation as described in Materials and Methods and tested statistically with a linear mixed-effects model. Factor is calculated as control divided by treated. The number n is the number of independent experiments and the number m is the total number of wells analysed (observations). pUC19 lin = linearized pUC19 plasmid. CpG ODN = short oligonucleotide rich in CG dinucleotide motifs. Control for Cytochalasin D (CytD)-treatment is with vehicle (DMSO).