Figure 3. SDS-PAGE analysis of expression of mmsB, PSEEN0851, and tsf in E. coli JM109.

The transformants were grown in LB at 37°C and induced by 1 mM IPTG. Lanes: M, molecular mass marker; 1, pQE-80L (as control); 2, pQE-mmsB; 3, pQE-PSEEN0851; 4, pQE-tsf. Arrowheads indicate the locations of recombinant proteins.