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. 2004 Feb 17;101(8):2311–2316. doi: 10.1073/pnas.0400073101

Fig. 1.

Fig. 1.

Specificity of the hAR-Np antiserum. (A) IP Western blots of a 4–20% SDS/PAGE gradient gel using the hAR-Np were detected at an ≈400-kDa band from extracts of MDCK, M1, IMCD3, and HEK293 cells. (B) Western blots using the hAR-C2p antiserum as IP and detected by hAR-C2m3C10. Bands were detected at ≈135 and ≈400 kDa from mouse tissues. MK, kidney; MLi, liver; MH, heart; MLu, lung. (C) Western blots of recombinant fusion proteins pCMV-Tag4hARF1–2. Lane a shows no band was detected by Flag-M2 in HEK293 cells without transfection (lanes a and d). Flag-M2 was reacted with an ≈105-kDa protein (lanes b and e) from the HEK293 cell with pCMV-Tag4hARF1–2 transfection. In lanes c and f, the same 105-kDa protein was also detected in extracts from the same transient transfected HEK293 cells by using hAR-Np and -Nm3G12 (lanes c and f). (D) Histological comparison of PKHD1 expression. (a) Pkhd1 antisense cRNA staining in the papillary collecting duct cells of the postnatal mouse kidney. (b) The PKHD1 protein expression is seen by using hAR-Np antiserum, which exhibits a similar staining pattern to the Pkhd1 mRNA pattern in the age-matched mouse kidney. The hAR-C2p (c) and -Cm3G6 (d) staining patterns in rat papillary tips are almost identical to those in mouse papillary staining (arrows) (b) (width, 25 μm in a–d).