Fig. 2.

PKHD1 tissue expression during mouse development. cRNA in situ hybridization of mouse embryos indicates that PKHD1 can be detected in the neural tube at E9.5 (A) and in bronchial buds (B) and epithelia of the midgut (C) at E10.5. Protein expression was also detected in each of the above organs, respectively, by using the hAR-Np (D–F). (F)4′,6-Diamidino-2-phenylindole (Sigma) for cell nuclei staining. At E11.5, PKHD1-positive staining is seen as the uretic bud projects into the metanephric primordium (right arrow, G) and was also observed in the mesonephric tubules (left arrows, G). At E15.5, PKHD1 appeared in cords of cells in the adrenal cortex (left arrow, H) and the liver (right arrow, H). Low and high powers of liver (I and J) show that PKHD1 is present in the cytoplasm of immature hepatic cells (arrows, I and J) (width, 1.5 μm, J; 10 μm, C, F, H, and I; 20 μm, A, B, D, and E; 30 μm, G).