Immunohistochemical evidence of 3-nitrotyrosine formation in the cortex of a rat kidney following ischemia/reperfusion (I/R). Panels refer to the following treatment groups: (A) negative control without primary antibody, (B) sham, (C) right nephrectomy control, (D) right nephrectomy + I/R, (E) right nephrectomy + L-N6-(1-iminoethyl)lysine + I/R, and (F) right nephrectomy + rutin + I/R. Magnification ×400.