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. Author manuscript; available in PMC: 2014 Mar 7.
Published in final edited form as: Dalton Trans. 2012 Dec 20;42(9):3100–3106. doi: 10.1039/c2dt32000b

Fig. 1.

Fig. 1

Iron binding of primary iron-sulphur cluster assembly proteins. Iron-sulphur cluster assembly proteins IscS, IscU, IscA, HscB and HscA were purified from E. coli cells grown in LB media. a), iron content of purified iron-sulphur cluster assembly proteins. Iron content was presented as the ratio of iron atoms per protein dimer. b), iron content of iron-sulphur cluster assembly proteins (50 μM in monomer) after incubation with Fe(NH4)2(SO4)2 (50 μM), sodium citrate (5 mM) and dithiothreitol (2 mM). Proteins were re-purified from incubation solutions. Iron content was presented as the ratio of iron atoms per protein dimer. The data are averages plus standard deviations from three independent experiments.