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. Author manuscript; available in PMC: 2014 Mar 7.
Published in final edited form as: Dalton Trans. 2012 Dec 20;42(9):3100–3106. doi: 10.1039/c2dt32000b

Fig. 5.

Fig. 5

In vitro iron-sulphur cluster assembly in IscA and IscA mutant Y40F. Purified IscA mutant Y40F (spectrum 1) or wild-type IscA dimer (spectrum 2) (25 μM) was incubated with IscS (0.5 μM), Fe(NH4)2(SO4)2 (100 μM), dithiothreitol (2 mM) in buffer containing Tris (20 mM, pH 8.0) and NaCl (200 mM) at 37°C under anaerobic conditions. L-cysteine (1 mM) was then added to the incubation solutions to initiate the iron-sulphur cluster assembly reaction. Spectra were taken 20 min after addition of L-cysteine. The absorption peak at 415 nm reflects the iron-sulphur cluster formation in IscA.