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Complementary activity of the IscA mutant Y40F in E. coli mutant with deletion of IscA and its paralog SufA. The E. coli wild-type (MC4100) and the mutant cells with deletion of IscA/SufA (iscA−/sufA−) containing expression plasmids were grown in M9 minimal media at 37°C under aerobic conditions. Cell growth was monitored at O.D. at 600 nm for 14 hours after inoculation of 1:100 dilutions.
