Table 1.

Experimental setup of all diffusion chambers throughout experimentation where number of experiments performed at each condition is indicated in the left column.

Condition (number)	Barrier	Bottom Chamber	Top Chamber
Negative control (2)	6–8 kDa membrane	200 μl of 5 mg/ml BSA	180 μl BSS
Positive control (2)	100 kDa membrane	200 μl of 5 mg/ml myoglobin	180 μl BSS
Corneal negative control (5)	Human cornea	200 μl of BSS	180 μl BSS
Corneal experimental variables (8)	Human cornea	200 μl of 5 mg/ml myoglobin	180 μl BSS
Corneal experimental variables (8)	Human cornea	200 μl of 5 mg/ml BSA	180 μl BSS