Table 1.
Gene | Exon | Primer sequence (5'-3') | Amplicon size (bp) | Annealing temperature (°C) |
---|---|---|---|---|
BMP2 | 2F | CTCACGTCGGTCCTGTCC | 392 | 53 |
2R | CCCTGCTCCATGCCTCAC | |||
3aF | CAAACGTCATTACTTGGCTTACTG | 591 | 51 | |
3aR | GCTGTTTGTGTTTGGCTTGA | |||
3bF | GCTAGTAACTTTTGGCCATGAT | 600 | 53 | |
3bR | TGTTTTCCCAACTTCTTCTTTTCG | |||
BMP4 | 3F | CCATCTTGCCCCTCCATTTCTA | 570 | 60 |
3R | CTTCTTCCCCAGGGCTTTCACT | |||
4aF | TGCTTATTTTCCCCCAGTAGGT | 704 | 60 | |
4aR | GGCGCCGGCAGTTCTTATTCTT | |||
4bF | GGGCCAGCATGTCAGGATTAGC | 575 | 60 | |
4bR | TGTGGGTGAGTGGATGGGAACG |