Fig. 5.

RACK1 modifies IP₃R function in response to muscarinic cholinergic and vasopressin stimulation. Free Ca²⁺ measurements were made in rat A7r5 and human A7r5 cells transfected for 60 h with YFP alone (black traces), YFP plus RACK1 (red traces), YFP plus siRNA (specific to cell species; blue traces), or YFP plus human siRNA plus rat RACK1 (purple traces). Ca²⁺ pools were released by vasopressin (Vaso, 10 nM; arrow) (A and B) in nominally Ca²⁺-free medium and carbachol (CCH, 100 μM; arrow) (C and D) in nominally Ca²⁺-free medium. All traces are averaged from multiple cells.