Table 1.
Details of Antibodies.
| Antigen | Dilution | Code, distributor | Host |
|---|---|---|---|
| VAMP-1 | 1 : 50 | 104 001, Synaptic Systems | Mouse |
| VAMP-2 | 1 : 2000 | VAS-SV006, Stressgen | Rabbit |
| VAMP-2 | 1 : 1000 | 104 211, Synaptic Systems | Mouse |
| VAMP-2 | 1 : 500 | V1389, Sigma-Aldrich | Rabbit |
| VAMP-3 | 1 : 100 | 104 102, Synaptic Systems | Rabbit |
| VAMP-4 | 1 : 100 | Ab3348, Abcam | Rabbit |
| SNAP-25 | 1 : 1000 | 567 343, Calbiochem | Rabbit |
| SNAP-25 | 1 : 2000 | VAM-SV012, Stressgen | Mouse |
| SNAP-25 | 1 : 2000 | S9684, Sigma-Aldrich | Rabbit |
| Syntaxin-1 | 1 : 200 | 110 302, Synaptic Systems | Rabbit |
| Munc-18 | 1 : 500 | 116 002, Synaptic systems | Rabbit |
| Munc-13 | 1 : 100 | 126 111, Synaptic Systems | Mouse |
| Synaptotagmin-1 | 1 : 1000 | AB5110, Millipore | Rabbit |
| Synaptotagmin-1 | 1 : 100 | 105 221, Synaptic Systems | Mouse |
| Synaptotagmin-7 | 1 : 200 | 105 172, Synaptic Systems | Rabbit |
| CAPS-1 | 1 : 200 | Kind gift of Professor Tom Martin (65) | Rabbit |
| Oxytocin | 1 : 500 | PS38, Kind gift of Professor Hal Gainer (66) | Mouse |
| Oxytocin | 1 : 200 | PC226L, Calbiochem | Rabbit |
| Vasopressin | 1 : 5000 | PS41, Kind gift of Professor Hal Gainer (66) | Mouse |
| Vasopressin | 1 : 400 | PC234L, Calbiochem | Rabbit |
| Chromogranin A+B | 1 : 100 | Ab8505, Abcam | Rabbit |
| LH-β | 1 : 200 | R23A, Kind gift of Professor Alan McNeilly (67) | Rabbit |
| Bassoon | 1 : 200 | 141 002, Synaptic systems | Rabbit |
| Synaptophysin | 1 : 1000 | 611 880, BD Biosciences | Mouse |
| GFAP | 1 : 1000 | IMG-5083A-1, IMGENEX | Rabbit |
| GFAP | 1 : 100 | IMG-5084A-1, IMGENEX | Mouse |
Manufacturers: Synaptic Systems: Goettingen, Germany; Stressgen: Enzo Life Sciences, Exeter, UK; Sigma-Aldrich: Dorset, UK; Abcam: Cambridge, UK; Calbiochem: Feltham, UK; Millipore: Watford, UK; BD Biosciences: Oxford, UK; IMGENEX: Upper Heyford, UK. Donors: Tom Martin: University of Wisconsin- Madison, Madison, WI, USA; Hal Gainer: NINDS Bethesda, MD, USA; Alan McNeilly: University of Edinburgh, Edinburgh, UK.