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. 2004 Feb 12;101(8):2351–2356. doi: 10.1073/pnas.0307337101

Fig. 4.

Fig. 4.

Bik is necessary for the fulvestrant-induced MCF-7/BUS cell apoptosis. (A) Suppression of Bik expression by siRNA. Cells were transiently transfected with Bik-specific (BIK) or sequence-scrambled control (CON) siRNA and then cultured in the presence or absence of 100 nM fulvestrant for 48 h, followed by evaluation of expression of Bik and actin (as control) by Western blotting. (B) Suppression of fulvestrant-induced cell death by Bik-specific siRNA. Cells were transiently transfected with Bik-specific (BIK) or sequence-scrambled control (CON) siRNA and then cultured in the presence or absence of 100 nM fulvestrant for 72 h. The phase-contrast images of the cultures show apoptotic cells that are detached from the dish and round-shaped. (C) Suppression of fulvestrant-induced PARP cleavage by Bik-specific siRNA. Cells were transiently transfected with Bik-specific (BIK) or sequence-scrambled control (CON) siRNA and then cultured in the presence or absence of 100 nM fulvestrant for 72 h. The full-length (116 kDa) and apoptosis-signature fragment (85 kDa) of PARP were detected by Western blotting.