Figure 3.

Odorant-evoked currents of the AgOr65 complex can be blocked by amiloride derivatives. (A–G) Representative whole-cell recordings of HEK cells expressing AgOr65 + AgOrco. After initial steady-state responses to 100 µM eugenol, increasing concentrations of the amiloride derivative were applied to each preparation. Holding potentials ranged from −60 to −50 mV, and the solutions were Extracellular 1 and Internal 1. (H) Inhibition curves for each of the amiloride derivatives, with data points representing the normalized mean ± SEM of the current reduction. IC₅₀ values and the number of trials (n) are in online supplementary Table S1. A color version of this figure appears in the online issue of Chemical Senses.