Figure 5.

HMA also blocks homomeric Orco channels from four insect species. (A–D) Whole-cell responses from cells expressing Orco channels from Anopheles gambiae (A, AgOrco), Harpegnathos saltator (B, HsOrco), Heliothis virescens (C, HvOrco), or Lygus hesperus (D, LhOrco) to an application of 100 µM VUAA1. HMA reduced VUAA1-mediated currents in a concentration-dependent manner. The holding potential for each recording was −60 mV, and the solutions were Extracellular 2 and Internal 2. (E) Inhibition curves for HMA and MIA against AgOrco or HsOrco, with data points representing the normalized mean ± SEM of the current reduction. IC₅₀ values and the number of trials (n) can be found in online supplementary Table S1. A color version of this figure appears in the online issue of Chemical Senses.