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. 2012 Dec 11;5(2):235–249. doi: 10.1002/emmm.201201846

Figure 6. Platelet MPs in RA SF exhibit autoantigens.

Figure 6

  • A. Identification of the autoantibodies contained in CD41+ mpICs in RA SF. The binding specificity of the mpIC-eluted IgG to 40 antigens was determined on a Bio-Plex™ bead-based antigen array and only the antigens that presented significant binding are presented (n = 23 RA patients, identified 1–23). Colours denote amount of binding: undetectable (blue); progressively greater binding (green, yellow, red). Color scale is presented on right. The negative control (C) was obtained by incubating isotypic antibodies in SF. Cfc; cyclic citrullinated filaggrin, H2A/a; histone H2A/a.
  • B–D. The presence of autoantigens was examined on in vitro platelet MPs (left) and platelet MPs from RA SF (right) by hs-FCM (n = 4). (B) MPs were incubated with anti-vimentin and the interaction was revealed using Alexa 647-conjugated secondary antibody. (C) MPs were incubated with exogenous Alexa 647-fluorescent fibrinogen and associations quantified by hs-FCM. (D) MPs were incubated with anti-fibrinogen and the interaction with endogenous fibrinogen was determined using Alexa 647-conjugated secondary antibody. The vertical lines were positioned according to the isotypic controls. Green: negative population; blue: positive population. The % of positive MPs is indicated on graphs.