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. 2012 Dec 11;5(2):235–249. doi: 10.1002/emmm.201201846

Figure 7. Platelet mpICs promote leukotriene production by neutrophils.

Figure 7

  1. Quantifications of the leukotriene biosynthesis by neutrophils incubated in presence of RA SF mpICs [n = 3 distinct patients ***p < 0.0001 patients 1 and 2; **p = 0.0011 patient 3 (unpaired t-test, relative to PBS control)]. Data are mean ± SEM. Leukotrienes measured represent the sum of LTB4, 6-trans-LTB4, 12-epi-6-trans-LTB4, 20-OH-LTB4 and 20-COOH-LTB4.
  2. Platelet mpICs form promptly upon co-incubation of platelet MPs with anti-fibrinogen in vitro. Representative FSC-PMT and SSC dot plot of mpICs. The presence of mpICs formed in vitro was demonstrated using a combination of FITC-conjugated Annexin-V and Alexa 647-conjugated secondary antibody. The four-quadrant gates were positioned according to the isotypic controls. The MPs (Annexin-V+-IgG−) presented in blue show dimensions ranging mostly from 100 to 500 nm (lower inset) while the mpICs (Annexin-V+-IgG+) presented in purple display dimensions ranging from 800 to 3000 nm (upper inset). The relative dimensions are presented according to size-defined microsphere calibrations.
  3. Quantifications of the leukotriene biosynthesis by neutrophils incubated in presence of in vitro mpICs, anti-fibrinogen and platelet MPs. The stimulatory potency of mpICs compared to the others conditions was statistically significant [n = 3 ***p ≤ 0.0001 (unpaired t-test)]. Data are mean ± SEM. Leukotrienes measured represent the sum of LTB4, 6-trans-LTB4, 12-epi-6-trans-LTB4, 20-OH-LTB4 and 20-COOH-LTB4.