Skip to main content
. 2012 Dec 11;5(1):38–51. doi: 10.1002/emmm.201201703

Figure 3. Self-renewal gene expression in CD34high and CD34low B-ALL blasts.

Figure 3

  1. Expression of TERT in CD34high and CD34low umbilical cord blood cells and leukaemic blasts. Umbilical cord blood shows higher expression of TERT in immature CD34high cells whilst no difference is seen between immature CD34high and CD34low blasts. TERT expression in blasts is comparable with, or higher than, that in CD34high umbilical cord blood progenitor cells. Expression values are 2-ΔCt using GAPDH as the reference gene. For purity of cells sorted for CD34 expression, see Supporting Information Fig S6.
  2. The PCA using a haematopoietic “self-renewal” signature containing 59 genes (213 probes) (Kim et al, 2009) also failed to separate CD34high and CD34low B-ALL populations (Supporting Information Fig S4). Here, we show the heatmap of the expression of 41 selected probes from this “self-renewal” signature.
  3. PCA plot generated using an AML “stem cell” signature (Eppert et al, 2011) that separates Lin-CD34highCD38low blasts (dark purple symbols) from more mature CD34low (pink symbols) blasts in AML (Gentles et al, 2010) but fails to detect any significant differences in “self-renewal” gene expression amongst the leukaemic subpopulations (black & white symbols) in B-ALL.