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. 2012 Dec 11;5(1):38–51. doi: 10.1002/emmm.201201703

Table 2.

Leukaemic engraftment at limiting dilution and quantification of LPC frequencies using unsorted B-ALL blasts and blasts sorted for expression levels of CD10, CD20 and CD34

Patient ID Transplant Transplanted blast population Number of engrafted mice/number of transplanted mice injected with Frequency of leukaemia-propagating cells estimate (confidence intervals)

1000 cells 100 cells 10 cells
Primary transplants
 EMCR1 Primary Unsorted 4/4 5/5 0/5 1:40 (1:16–112)
 EMCR2 Primary Unsorted 2/3 0/5 0/5 1:1200 (1:300–4700)
 L858 Primary Unsorted 4/5 2/5 0/5 1:460 (1:180–1200)
 L876 Primary Unsorted 4/4 4/5 0/5 1:80 (1:30–210)
 2003 Primary Unsorted 1/3 0/4 0/5 1:2900 (1:420–20,000)
 4917 Primary Unsorted 1/3 0/4 0/5 1:2900 (1:420–20,000)
 L4967 Primary Unsorted 4/4 5/5 0/5 1:40 (1:16–110)
L49120 Primary Unsorted 1/4 1/5 0/5 1:1900 (1:540–6600)
L49101 Primary Unsorted 2/4 0/4 0/4 1:950 (1:240–3700)
Secondary/tertiary transplants (primografts)
 L4951 Secondary Unsorted 6/6 10/10 5/8 1:10 (1:4–25)
L49101 Secondary Unsorted 4/4 5/5 4/5 1:6 (1:2–18)
 L4967 Tertiary Unsorted 3/3 5/5 3/5 1:11 (1:3–35)
CD10
 EMCR1 Secondary CD10high 4/4 2/4 0/5 1:170 (1:47–590)
CD10low 4/4 2/3 0/5 1:120 (1:30–460)
CD20
 EMCR1 Primary CD20high 4/4a Unable to calculate
CD20low 4/4a Unable to calculate
 EMCR1 Secondary CD20high 4/4 2/4 0/5 1:170 (1:47–590)
CD20low 2/4 0/4 0/5 1:1700 (1:420–6700)
 2510 Secondary CD20high 2/4 0/4 1:4900 (1:1200–20,000)
CD20low 4/4 1/4 1:780 (1:230–2700)
 L4951 Secondary CD20high 11/11 5/5 Unable to calculate
CD20low 10/11 6/6 Unable to calculate
Tertiary CD20high 3/3 0/4 1:140 (1:25–740)
CD20low 3/3 0/4 1:140 (1:25–740)
CD34
 L784 Primary CD34high 3/4 0/5 0/5 1:930 (1:300–2.900)
CD34low 3/4 1/5 0/6 1:660 (1:230–1900)
 4540a Primary CD34high 2/5 3/5 0/4 1:840 (1:300–2.300)
CD34low 1/5 0/5 0/5 1:5.000 (1:710–36.000)
 EMCR1 Secondary CD34high 4/4 1/5 3/5 1:120 (1:40–360)
CD34low 4/4 2/5 1/5 1:140 (1:46–450)
 L4951 Tertiary CD34high 3/3 5/5 5/6 1:6 (1:2–15)
CD34low 4/4 5/5 4/5 1:6 (1:2–1:18)
a

Primary cells from patient EMCR1 were transplanted at a cell dose of 1500 cells/mouse. Group sizes >5 indicate pooled data from several transplantation experiments.