Table 2.
Patient ID | Transplant | Transplanted blast population | Number of engrafted mice/number of transplanted mice injected with | Frequency of leukaemia-propagating cells estimate (confidence intervals) | ||
---|---|---|---|---|---|---|
1000 cells | 100 cells | 10 cells | ||||
Primary transplants | ||||||
EMCR1 | Primary | Unsorted | 4/4 | 5/5 | 0/5 | 1:40 (1:16–112) |
EMCR2 | Primary | Unsorted | 2/3 | 0/5 | 0/5 | 1:1200 (1:300–4700) |
L858 | Primary | Unsorted | 4/5 | 2/5 | 0/5 | 1:460 (1:180–1200) |
L876 | Primary | Unsorted | 4/4 | 4/5 | 0/5 | 1:80 (1:30–210) |
2003 | Primary | Unsorted | 1/3 | 0/4 | 0/5 | 1:2900 (1:420–20,000) |
4917 | Primary | Unsorted | 1/3 | 0/4 | 0/5 | 1:2900 (1:420–20,000) |
L4967 | Primary | Unsorted | 4/4 | 5/5 | 0/5 | 1:40 (1:16–110) |
L49120 | Primary | Unsorted | 1/4 | 1/5 | 0/5 | 1:1900 (1:540–6600) |
L49101 | Primary | Unsorted | 2/4 | 0/4 | 0/4 | 1:950 (1:240–3700) |
Secondary/tertiary transplants (primografts) | ||||||
L4951 | Secondary | Unsorted | 6/6 | 10/10 | 5/8 | 1:10 (1:4–25) |
L49101 | Secondary | Unsorted | 4/4 | 5/5 | 4/5 | 1:6 (1:2–18) |
L4967 | Tertiary | Unsorted | 3/3 | 5/5 | 3/5 | 1:11 (1:3–35) |
CD10 | ||||||
EMCR1 | Secondary | CD10high | 4/4 | 2/4 | 0/5 | 1:170 (1:47–590) |
CD10low | 4/4 | 2/3 | 0/5 | 1:120 (1:30–460) | ||
CD20 | ||||||
EMCR1 | Primary | CD20high | 4/4a | – | – | Unable to calculate |
CD20low | 4/4a | – | – | Unable to calculate | ||
EMCR1 | Secondary | CD20high | 4/4 | 2/4 | 0/5 | 1:170 (1:47–590) |
CD20low | 2/4 | 0/4 | 0/5 | 1:1700 (1:420–6700) | ||
2510 | Secondary | CD20high | 2/4 | 0/4 | – | 1:4900 (1:1200–20,000) |
CD20low | 4/4 | 1/4 | – | 1:780 (1:230–2700) | ||
L4951 | Secondary | CD20high | 11/11 | 5/5 | – | Unable to calculate |
CD20low | 10/11 | 6/6 | – | Unable to calculate | ||
Tertiary | CD20high | – | 3/3 | 0/4 | 1:140 (1:25–740) | |
CD20low | – | 3/3 | 0/4 | 1:140 (1:25–740) | ||
CD34 | ||||||
L784 | Primary | CD34high | 3/4 | 0/5 | 0/5 | 1:930 (1:300–2.900) |
CD34low | 3/4 | 1/5 | 0/6 | 1:660 (1:230–1900) | ||
4540a | Primary | CD34high | 2/5 | 3/5 | 0/4 | 1:840 (1:300–2.300) |
CD34low | 1/5 | 0/5 | 0/5 | 1:5.000 (1:710–36.000) | ||
EMCR1 | Secondary | CD34high | 4/4 | 1/5 | 3/5 | 1:120 (1:40–360) |
CD34low | 4/4 | 2/5 | 1/5 | 1:140 (1:46–450) | ||
L4951 | Tertiary | CD34high | 3/3 | 5/5 | 5/6 | 1:6 (1:2–15) |
CD34low | 4/4 | 5/5 | 4/5 | 1:6 (1:2–1:18) |
Primary cells from patient EMCR1 were transplanted at a cell dose of 1500 cells/mouse. Group sizes >5 indicate pooled data from several transplantation experiments.