Skip to main content
. 2012 Nov 25;5(1):105–121. doi: 10.1002/emmm.201201283

Figure 2. I2PP2A/SET is overexpressed and C18-ceramide is decreased in primary lung tumour tissues.

Figure 2

  1. Ten paired samples of fresh frozen lung adenocarcinoma tumours (T) with adjacent, non-cancerous lung tissues (N) were homogenized and Western blotting was done to evaluate I2PP2A/SET. β-actin was used as a control. Full blots can be seen in Supporting Information Fig S15.
  2. I2PP2A/SET was detected in N7, N5 versus T7, T5 tissues using IHC (left and right panels, respectively).
  3. Ceramides were measured by LC/MS/MS in N1–10 versus T1–10 tissues (normalized to µg of protein), and C18-ceramide levels in N1–10 compared to T1–10 tissues were reported.
  4. CerS1 and CerS6 mRNA were measured by Q-PCR in T1–10 compared to N1–10 tissues (normalized to rRNA).
  5. I2PP2A/SET was detected by IHC and scored (percentage of I2PP2A/SET expression was calculated using positive staining scored between 1 and 5 and intensity of staining scored between 1 and 3) in TMAs containing lung tumour (1) and non-cancerous lung tissues (2) (n = 48 pairs), and normal lung tissues (n = 4). p < 0.05 was considered significant (calculated using paired t-test).