Table 1.
Binding of purified human I2PP2A/SET to C18-ceramide, FTY720 and P-FTY720 in vitro, measured using SPR
| Surface | Protein | Protein Kd (nM) |
|---|---|---|
| POPC:POPE | I2PP2A | 70 ± 20 |
| C18-ceramidea | I2PP2A | 11 ± 7 |
| FTY720a | I2PP2A | 11 ± 9 |
| FTY720P | I2PP2A | N.D.b |
| POPC:POPE | p47phox-PX | N.D. |
| C18-ceramidea | p47phox-PX | N.D. |
| FTY720a | p47phox-PX | N.D. |
Kd values determined with SPR. All data was collected at 1 µM I2PP2A in 20 mM HEPES, pH 7.4 buffer containing 160 mM KCl.
a
Liposomes contained POPC:POPE:x at (75:20:5) ratio while control liposomes contained POPC:POPE at a (80:20) ratio.
b
N.D. refers to not detectable on the indicated surface.