Figure 6. Inhibition of aurora kinases induces senescence, DNA damage and SASP in patient tumour implants treated with MLN8237.

1. The tissue level of senescence in a melanoma patient implant (V35) after mice were treated with MLN8237 or vehicle was determined by β-galactosidase staining.
2. To assess DNA damage, 53BP1 (red) was visualized by immunofluorescence and tissues were co-stained with α-tubulin (green) and DAPI (blue). A and B were analysed from multiple slides and representative images are shown.
3. Cytokine profile of MLN8237-treated tumour tissue was analysed in tumour tissue lysates using cytokine array.
4. The infiltrating neutrophils and macrophages were evaluated by FACS using anti-Ly-6G and F4/80 antibodies, respectively. Seven tumours were analysed from each group. Means ± SEM are shown. p-value: ****9.16945E−06; ***0.000573.