Table 5.

List of differentially expressed proteins obtained by using proteomic analysis.

	Accession number	Spot numbera	Putative function	Proteomic ratio of biofilm/planctonic conditionsb	RNA-seq ratio of biofilm/planctonic conditionsc
1	AFUA_1G05340	38	40s ribosomal protein S19	0.43	4.21
2	AFUA_1G07480	15	Coproporphyrinogen III oxidase	0.39
3	AFUA_1G12890	20	Probable 60s ribosomal protein l5	0.40
		22		0.40
4	AFUA_1G14120	11	Nuclear segregation protein (Bfr1)	0.47
5	AFUA_1G16840	32	TCTP family protein	0.43	2.96
6	AFUA_2G04060	15	NADH:flavin Oxidoreductase/NADH oxidase family protein	0.39	0.14
7	AFUA_2G07420	6	Actin-bundling protein Sac6	0.39
8	AFUA_2G11010	9	Dihydroorotate reductase PyrE	0.15
		10		0.37
9	AFUA_2G11150	12	Secretory pathway gdp dissociation inhibitor	0.49
10	AFUA_3G00590	41	Asp-hemolysin	0.36
11	AFUA_3G08420	34	Cystathionine beta-synthase (beta-thionase)	0.45
12	AFUA_3G12300	39	60s ribosomal protein L22	0.37	5.60
13	AFUA_4G03410	13	Flavohemoprotein	0.28	0.05
		14		0.32
14	AFUA_4G08240	19	Zinc-containing alcohol dehydrogenase	0.47	0.05
15	AFUA_4G11080	40	Acetyl-coenzyme A synthetase FacA	2.40	0.29
16	AFUA_5G02370	6	Vacuolar ATP synthase catalytic subunit A	0.39
17	AFUA_5G06240	18	Alcohol dehydrogenase, putative	0.24	0.15
		23		0.35
18	AFUA_5G09910	33	Nitroreductase family protein	0.38	0.12
19	AFUA_5G14680	29	Conserved hypothetical protein	0.20	0.25
		30		0.32
		31		0.14
20	AFUA_6G02420	43	Ubiquitin conjugating enzyme (UbcM)	2.01	4.77
21	AFUA_6G02750	36	Nascent polypeptide-associated complex (NAC) subunit	0.42	2.09
22	AFUA_6G05210	27	Malate dehydrogenase, NAD-dependent	2.12
23	AFUA_6G07430	7	Pyruvate kinase	0.45	0.43
24	AFUA_7G01010	19	Alcohol dehydrogenase	0.47	83.45
25	AFUA_8G03930	3	Hsp70 chaperone (HscA)	0.46	0.46
		4		0.49
		5		0.45

^aSpot number in Figure 4.

^bAverage ratios compared under biofilm and planctonic growth conditions were extracted from statistical analysis of DIGE gels by the Delta2D 4.3 (Decodon) software program. “>2” a consistent increase of greater than twofold. “<2” a consistent decrease of more than twofold.

^cThe transcriptional changes determined by RNA-seq are aligned.