Fig. 1.

pIL-1α is translocated to the nucleus upon inflammatory stimuli. NIH/3T3 cells (20,000 per well) were plated on a cover slip placed in six-well plates. Twenty-four hours later, cells were transfected with both the vectors encoding the fusion proteins pIL-1α/EYFP-N1 and pIL-1α/ECFP-C1 (a). Twenty-four hours after transfection, an aliquot of cells were stimulated with lipopolysaccharide (10 μg/ml). Twenty-four hours later, cover slips were observed by fluorescent microscopy. (a) Fusion proteins used (calpain cleavage site marked by arrow). (b and c) Unstimulated cells observed through yellow and cyan filters, respectively. (d–f) Stimulated cells observed through light microscopy and yellow and cyan filters, respectively.