Fig. 3.

IL-1α activates transcription from a heterologous promoter. MS1 cells were transfected with empty plasmid (mock) or plasmids expressing the GAL4 DBD, either alone or upstream of pIL-1α, mIL-1α, or ppIL-1α, represented by pIL-GAL4, mIL-GAL4, and ppIL-GAL4, respectively. Cells were also cotransfected with pG5-LUC containing five copies of the GAL4 enhancer site upstream of the luciferase gene and with pNULL-Renilla to control for transfection efficiency. Data represent the mean ± SEM of three separate experiments, each performed in triplicate.