Fig. 4.

Recombinant IL-1Ra abrogates activity of endogenous IL-1α released to supernatants. Raw264.7 cells were stably transfected with either pcDNA6/His-myc (vector) or pIL-1α expression vector. Cells were plated in the presence of 10 μg/ml IL-1Ra as indicated. On the next day, cells were washed with PBS and replenished with medium containing nothing (control), A23187, IL-1Ra, or both A23187 and IL-1Ra. Twenty-four hours later, medium was removed and concentrations of MIP-2 were measured by ECL.