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Expression of intracellular IL-1α augments MS1 cells responses to IFN-γ and TNF-α. Stable, pooled MS1 cells transfected with either vector or expression vectors for pIL-1α or pIL-1β were plated in the presence of saturating concentrations of IL-1Ra (10 μg/ml). Twenty-four hours later, cells were washed and treated with IL-1Ra together with either TNF-α (Upper) or IFN-γ (Lower). After 24 h, MIP-2 was measured. Data represent the mean ± SEM of three separate experiments.
