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Transcription across the Th2 cytokine cluster in human fibroblasts. RNA was isolated from resting or activated normal skin fibroblasts and subjected to RT-PCR for the Th2 cytokine cluster genic (A) and intergenic (B) transcripts. Where indicated (+Act) cells were activated with IL-1β, tumor necrosis factor α, and IFN-γ for 24 h. Number of PCR cycles: β-actin, 22; hIL-4, 34; hIL-13, 34; RAD50, 27; KIF3A, 27; GM-CSF, 32; IG1–5, 34.
