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. Author manuscript; available in PMC: 2014 Feb 6.
Published in final edited form as: Neuron. 2013 Feb 6;77(3):542–558. doi: 10.1016/j.neuron.2012.11.029

Figure 2. Postsynaptic Stx-3 KD in vivo Does Not Alter Basal Synaptic Transmission or Constitutive Recycling.

Figure 2

(A) Merged images of dendrites stained for endogenous Stx-3 and PSD95. Right panel shows enlargement of single dendrite (box in left panel). See also Movie S1. (B–C) Mean amplitude (B) and frequency (C) of mEPSCs are unchanged by the Stx-3 KD. Cumulative probability graphs of mEPSC amplitudes and frequencies are shown on left and summary graphs (means ± SEM) on the right. (D) The ratio of AMPAR- to NMDAR-mediated EPSCs is unchanged by the Stx-3 KD. Representative EPSCs recorded at −60 mV and + 40 mV are shown with scatter plots of individual recordings. Different colored points indicated mean ± SEM. Scale bar represents 50 ms/25 pA. (E) Voltage-dependence of NMDAR EPSCs is not affected by Stx-3 KD. (F) Representative images and summary graphs showing endosome recycling. Alexa 488-transferrin (Alx-Tf) uptake at time=0 and after 20 min recycling was measured in control and Stx-3 KD cultured neurons. Loss of Alx-Tf reflects recycling. Bar graphs represent means ± SEM. See also Figure S3.