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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Immunity. 2013 Jan 11;38(1):176–186. doi: 10.1016/j.immuni.2012.11.011

Table 2.

Effect of Amino Acid Substitutions in the V2 Region of HIV-1 Env on Ability of HIV-1 V2 mAbs to Bind. See Also Table S4.

mAb EC50, ug/ml
A244 gp120 AE.703359 gp120 AE.427299 gp120 gp70 B.CaseA2 V1–V2
WT K169V Mut3 WT Q169K Mut3 WT Q169K Mut4 WT V169K Mut3
RV144V2 antibodies:
 CH58 0.03 0.05 7 0.38 0.04 0.07 22.5 3.4 0.03 1.2 0.025 0.03
 CH59 0.029 0.58 NB NB 1.2 0.05 NB NB 0.03 NB NB 0.02
Other V1V2 antibodies:
 697D 0.1 0.06 0.08 0.21 0.51 0.12 0.07 0.13 NB 0.04 0.14 0.04
 PG9 0.6 3 >100 >5 0.53 0.54 0.47 0.13 >10 NB >100 >10
 PG16 >10 >100 NB NB >10 >10 >10 0.26 NB NB NB NB
 CH01 0.35 0.13 0.93 NB NB NB >10 >10 NB NB NB NB

The indicated HIV-1 Env willdtype (WT), and Env mutants with a single a.a residue substitution at the position 169, 3 a. a. residue substitutions (Mut3) or 4 a. a. residue substitutions (Mut4) were assayed in ELISA.

AE.A244 gp120/Mut 3 = K169V/V172E/H173Y.

AE.703359 gp120/Mut 3 = Q169K/R170Q/Q173H.

AE.427299 gp120/Mut 4 = R168K/Q169K//Y173H/A174V.

gp70 B.CaseA2 V1–V2/Mut3 = V169K/E172V/E173H.