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Activation of endothelial caspase pathways. (A) Dose-dependent activation of caspase-3 in adiponectin-treated BCE cells at 24 h. (B–D) Time course determination of activation of caspase-3-like (B), caspase-8-like (C), and caspase-9-like (D) enzymes in BCE cells treated with 1 μg/ml human adiponectin. Activation of caspase-3 at 3 or 6 h (E and G), caspase-9 at 6 h (F and H), and caspase-8 at 6 h (H) was determined in the presence or absence of specific caspase inhibitors.
