Table 1. β-Galactosidase expression by E. coli strains carrying wild-type and mutant forms of DtxR grown under iron-replete and iron-limiting conditions.
| β-Galactosidase, % of control
|
||
|---|---|---|
| Repressor | LB/Amp/Kan | LB/Amp/Kan + 300 μM DP |
| DtxR | 0.32 ± 0.04 | 81.0 ± 2.25 |
| DtxR(E175K) | 0.25 ± 0.04 | 30.7 ± 1.61 |
| DtxR(G10) | 0.76 ± 0.07 | 95.9 ± 3.41 |
| DtxR(G4S)2 | 0.43 ± 0.12 | 97.5 ± 5.62 |
| DtxR(E175K,G10) | 0.65 ± 0.73 | 22.3 ± 0.42 |