Table 4. β-Galactosidase expression by E. coli strains carrying wild-type and mutant forms of DtxR with various amino acids at position 175 grown under iron-replete and iron-limiting conditions.
| β-Galactosidase, % of control
|
||
|---|---|---|
| Amino acid at position 175 | LB/Amp/Kan | LB/Amp/Kan + 300 μM DP |
| E* | 0.24 ± 0.12 | 48.4 ± 2.83 |
| D | 0.41 ± 0.23 | 48.7 ± 2.92 |
| K | 0.20 ± 0.10 | 8.71 ± 0.25 |
| R | 0.16 ± 0.03 | 8.93 ± 1.08 |
| Q | 0.14 ± 0.01 | 21.2 ± 0.31 |
| A | 0.12 ± 0.05 | 24.6 ± 1.61 |
Wild type