Fig. 3.

JESEBL/EBA-181 variants recognize different erythrocyte receptors. Region II of JESEBL/EBA-181 expressed in CHO-K1 cells or COS7 (RVNQN) cells contains the mutations shown at the positions delineated in Table 1. Transfected cells with five or more attached erythrocytes were counted, and the total per coverslip was recorded. The transfection efficiency of all constructs into CHO-K1 cells was 80–90%. Data are shown as the mean of three independent experiments, and the error bar denotes the SE. Data from enzyme-treated erythrocytes are expressed as the percentage of binding to untreated erythrocytes. The control normal untreated erythrocyte samples contained between 100 and 198 cells with bound erythrocytes. Arrowheads and diamonds represent point mutations (see Table 1).