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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Methods. 2012 Apr 4;57(3):272–279. doi: 10.1016/j.ymeth.2012.03.024

Fig. 2.

Fig. 2

Schematic of a Raman flow cytometer. Excitation is provided by a solid state laser (660 nm, 400 mW) and forward angle light scatter is collected on a photodiode. Ninety degree light scatter is collected from one side of the flow cell via an optical fiber and detected with a photomultiplier tube (PMT). SERS signals are collected from the opposite side of the flow cell into and optical fiber and delivered to an imaging spectrograph coupled to a CCD detector. Particles in the probe volume are detected by forward and side scatter, which trigger the acquisition of individual particle spectra by the CCD.