FIG 7 .

Growth and bacterial cell counts of PA14 WT determined using various amino acids as sole nitrogen (N) sources. (A) P. aeruginosa bacteria were grown in liquid MSCFM supplemented with total amino acids (control) or single amino acids serving as the sole N source. Growth was measured at 37°C using a Tecan Spectrofluor Plus reader. (B) P. aeruginosa bacteria were spotted onto MSCFM agar-mucin plates supplemented with total amino acids (control) or a single sole N source (as indicated) and incubated at 37°C for 13 h. The entire surface motility colony was resuspended in 1× PBS and serially plated on LB agar plates. Results shown are means ± SD for at least three independent experiments with duplicates for each experiment. Asterisks indicate a statistically significant difference (P ≤ 0.05) between the sole nitrogen source and control as determined by Student’s t test.