Fig. 5.
Titration of F-spondin-mediated inhibition of APP cleavage by BACE 1. (A) Relative levels of proteins expressed in an experiment similar to that described in Fig. 4. Increasing amounts of Ig-F spondin plasmid were cotransfected with constant amounts of APP and BACE 1. The levels of full-length APP and the CTFs of APP and of F-spondin were quantified by immunoblotting and are shown in arbitrary units. (B) Ratio of CTF to full-length APP as a function of increasing amount of F-spondin. CTF levels were corrected for APP expression. Data shown are means ± SEM from a representative experiment (n = 3) independently repeated multiple times.