FIG. 2.

Calibration curve of Hoechst 33342-saturated zebrafish Z3 cells seeded at various cell densities. Hoechst fluorescence was recorded after 80-min incubation with the Hoechst 33342 nuclear stain (1 μg/mL) in Z3 cells which were seeded at various densities in triplicates 24 h earlier. The curve fitted dashed line has a correlation factor R²=0.994. Inset: The incubation time until Hoechst fluorescence signal becomes saturated. Fluorescence was recorded every 10 min after incubation started in triplicate wells holding 5×10⁴ zebrafish Z3 cells/cm². The dotted line marks the 80-min incubation time point. All values represent blank-corrected means±SEM of three independent cell culture preparations.