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Viability and phase contrast microscopy of HepG2s encapsulated in alginate beads. (A, D) Fluorescein diacetate (FDA)-stained viable cells. (B, E) Propidium iodide (PI)-stained dead cells. (C, F) Phase contrast images. (A–C) Day of encapsulation, (D–F) after 10 days of proliferation in fluidized bed bioreactor.
