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Metabolite levels during culture of proliferating biomass. (A) Glucose concentration during proliferation phase (n=5, mean±SEM), peaks match media changes on days 4, 6, 8, and 10). (B) Lactate profile: Lactate concentration during proliferation (n=5, mean±SEM). Falls in lactate correspond to media changes (days 4, 6, 8, and 10). (C) Alpha-fetoprotein (AFP) concentration; vertical lines represent samples before and after media change.
