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miR-200a or miR-200b mimics were transfected into human endometrial cancer cell line HEC-1A cells. (a) qRT-PCR assay showed TIMP2 mRNA expression was significantly reduced only in cells transfected with the miR-200b mimic. (b, c) Enzyme-linked immunosorbent assay and western blot assay showed TIMP2 protein expression was specifically reduced following transfection of the miR-200b mimic. *P=0.05.
