Figure 3.

GrM cleaves hnRNP K more efficiently in the presence of RNA. (a) Jurkat cell lysates were pretreated with RNase (100 μg/ml) or left untreated for 30 min at 37°C, followed by incubations with increasing concentrations of GrM or GrM-SA (300 nM) for 4 h at 37°C and immunoblotted for hnRNP K or α-tubulin (upper panel). Band intensities were quantified and depicted in graphs (lower panel). (b) Purified His-hnRNP K (1.5 μg) was pretreated with RNA (100 ng) or left untreated, followed by incubations with increasing concentrations of GrM or GrM-SA (300 nM) for 16 h at 37°C. Samples were subjected to SDS-PAGE and gels were stained with InstantBlue (upper panel). Band intensities were quantified and depicted in graphs (lower panel). Data depicted are representative of at least two independent experiments