Figure 6.

Effects of low pH, PPIs, and cholesterol inhibitors in blebbishield-mediated sphere formation (a) Effect of pH on sphere formation from blebbishields. Blebbishields isolated at pH 7.5 were plated in indicated pH for sphere formation. (b) Effect of pH on blebbishield formation (histogram on left) and determining the survival capacity of blebbishields from these conditions in normal pH 7.5 (histogram on right). (c) Proton pump v-ATPase inhibitor reduces sphere formation in the presence of HCl. (d) Proton pump v-ATPase inhibitor reduces sphere formation in the presence of lactic acid. Right panel shows visual indication of stress induced by esomeprazole and schematic diagram. (e) Filipin-III (freshly prepared) abolishes the lactate induced high frequency sphere formation. Photomicrographs shows that Filipin-III by itself is not toxic to RT4P cells