Figure 7.

SIRT2 upregulates Aurora A expression. (a) BE(2)-C and MiaPaca-2 cells were transfected with scrambled control siRNA, SIRT2 siRNA-1 or SIRT2 siRNA-2 for 48 h, followed by real-time RT-PCR and immunoblot analyses of Aurora A mRNA and protein expression. (b and c) BE(2)-C and MiaPaca-2 cells were treated with vehicle control, the SIRT2 inhibitor AC-93253 at 0.8 μM (b), or Salermide at 50 μM (c) for 48 h, followed by real-time RT-PCR and immunoblot analyses of Aurora A mRNA and protein expression. Error bars represented standard error. *P<0.05