Fig. 3.

Effect of cGMP, KT5823 on TRPC3-mediated store-operated Ca²⁺ influx in HEK293 cells without PKG transfection. 8-BrcGMP (2 mM) and KT5823 (1 μM) had no effect on the store-operated Ca²⁺ influx in the HEK293 cells that were transiently transfected with TRPC3 (A) or in wild-type HEK293 cells (B). Cells were not transfected with the PKG gene. Cells were depleted with 4 μM thapsigargin in 0 Ca²⁺-PSS for 15 min. Control had no thapsigargin treatment. 8-BrcGMP with or without KT5823 was introduced 5 min before the experiments. At the time indicated by the arrow, the media were changed to 0.5 Ca²⁺-PSS. Shown is the mean ± SE (n = 8 experiments).