Figure 1.

Serum deprivation or modification of serum content differently affects the effect of cannabinoids (CBD, CBG, CBC) on the viability of human PCCs in the MTT assay. (A) Cells were grown in presence of 10% FBS in six-well dishes. After adhesion, cells were serum-deprived (with or without 0.5%BSA) for 16 h and subsequently treated with compounds for 24 h. Cell viability was assessed by the MTT assay. Data shown are means ± SEM of % inhibition of MTT-reducing activity, calculated from three independent experiments. ***P < 0.001, FBS-free vs. plus 0.5% BSA; anova followed by Bonferroni's test. (B) Cells grown in presence of 10% FBS were incubated, after adhesion, under different conditions (a–c) in six-well dishes for 72 h: (a) cells were incubated in presence of 10% FBS; (b) cells were incubated with 10% FBS that had been protein-depleted; (c) cells were incubated again in 10% FBS that had been protein-depleted, but supplemented with 0.5%BSA. Cell viability was assessed by the MTT assay. Data shown are means ± SEM of % inhibition of MTT-reducing activity calculated from three independent experiments. ###P < 0.001 protein-depleted serum [b] vs. 10% FBS [a] and protein-depleted serum plus 0.5% BSA [c]; $$P < 0.01 protein-depleted serum plus 0.5% BSA [c] vs. 10% FBS [a]; anova followed by Bonferroni's test. (C) Cells were grown in 10% FBS in six-well dishes. After adhesion, cells were serum-deprived for 16 h and then treated with compounds for 24 h. Cell viability was assessed by the MTT assay. Data shown are means ± SEM of % inhibition of MTT-reducing activity calculated from three independent experiments.