Table 2.
Effect of plant cannabinoids on the viability of human prostate carcinoma androgen receptor-positive (LNCaP) cells
| Pure compound | IC50 (µM) on cell viability [A] | IC50 (µM) on cell viability [B] | BDS | IC50 (µM) on cell viability [A] | IC50 (µM) on cell viability [B] |
|---|---|---|---|---|---|
| CBD | 25.0 ± 3 | 5.7 ± 2 | CBD BDS | 18.1 ± 6 | 6.6 ± 2 |
| CBC | 20.0 ± 5 | 10.9 ± 3 | CBC BDS | >25 (25.6%) | 7.9 ± 1 |
| CBG | >25 (34.5%) | 11.2 ± 4 | CBG BDS | 21 ± 8 | 9.0 ± 1 |
| CBDV | >25 (27.6%) | 20.0 ± 3 | CBDV BDS | >25 (24.4%) | 10.4 ± 1 |
| THCV | >25 (28.5%) | 17.5 ± 3 | THCV BDS | 16.3 ± 5 | 7.2 ± 1 |
| THCVA | >25 (32.4%) | 11.5 ± 5 | THCVA BDS | 19.4 ± 8 | 5.6 ± 1 |
| THCA | 22.1 ± 2 | 17.1 ± 1 | THCA BDS | 15.0 ± 2 | 4.0 ± 3 |
| CBDA | >25 (30.2%) | 16.2 ± 5 | CBDA BDS | >25 (34.5%) | 9.3 ± 2 |
| CBGA | >25 (7.0%) | 11.6 ± 2 | CBGA BDS | 14.5 ± 2 | 8.5 ± 2 |
| CBGV | >25 (23.9%) | >25 (41.0%) | CBGV BDS | >25 (40.2%) | 9.4 ± 2 |
| CBN | 14.5 ± 6 | >25 (34.2%) | – | – | – |
| THC | 16.9 ± 3 | 5.5 ± 3 | – | – | – |
(A) Cells were seeded in presence of 10% FBS in six-well Multiwell with a density of 1 × 105 cells·per well. After adhesion, cells were treated with increasing concentrations of compounds for 72 h (presence of serum was maintained during the treatments). (B) Cells were seeded in presence of 10% FBS in six-well Multiwell with a density of 1 × 105 cells·per well. After adhesion, cells were starved for 16 h and subsequently treated with increasing concentrations of compounds for 24 h (absence of serum was maintained during the treatments). Cell viability was assessed by MTT staining. Data are reported as mean ± SD of IC50 values calculated from three independent experiments. In the case of IC50 > 25 µM, the maximum inhibition observed at the highest concentration tested (25 µM) is shown.