Figure 6.

Effects of escitalopram on ex vivo noradrenaline and 5-HT uptake by low-affinity transporter in the frontal cortex in WT mice. (A–C) Representative [³H]noradrenaline and 5-HT uptake in cortex extracts in the presence of increasing concentrations of escitalopram (ESC; 1 µM to 10 mM) with or without the inhibitor D22 is expressed as mean fmol ± SEM, normalized by protein concentration (n= 3–5 experiments). (B–D) D22-sensitive [³H]noradrenaline and 5-HT uptake in the presence of increasing concentrations of escitalopram (100 µM to 10 mM) is expressed as percentage of D22-sensitive uptake in absence of citalopram (Control; n= 3–5). D22-sensitive uptake rates were defined by subtracting uptake in the presence of D22 from the uptake observed in its absence. *P < 0.05; **P < 0.01: significantly different from control (one-way anova followed by Fisher's post hoc test).