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Calcium mobilization experiments. Concentration–response curves to N/OFQ, [Dmt1]N/OFQ(1–13)-NH2, and standard opioid agonists (dynorphin A and DPDPE for κ and δ opioid receptors, respectively) in CHO cells stably expressing chimeric G proteins and the κ (left panel) and δ (right panel) human recombinant opioid receptor. Data are the mean ± SEM of four separate experiments performed in duplicate.
