Figure 4.

GEPO exhibits no effect on rHuEPO-induced proliferation of UT-7 cells. (A) UT-7 cells were cultured in semisolid medium with or without either 0.03 μg mL⁻¹ of rHuEPO or GEPO. The colony count of rHuEPO-treated UT-7 cells at day 7 and day 14 was significantly higher than that of GEPO treated and untreated cells. In contrast, the colony count of GEPO treated UT-7 was not different from the untreated cells. (*P < 0.05 vs. untreated cells, ŧP < 0.05 vs. GEPO treated cells). (B) MTT proliferation assay results after culturing UT-7 cells in 10% FBS DMEM supplemented with and without either rHuEPO or GEPO also showed that the proliferation rate of rHuEPO-treated UT-7 cells was markedly higher than that of GEPO-treated and untreated cells. Notably, the proliferation rate of UT-7 cells that were pretreated with GEPO was increased by adding rHuEPO to culture media. (*P < 0.05 vs. untreated cells, ^ŧP < 0.05 vs. GEPO-treated cells)